VOL 30 *No.1http://www.pesting.org.rs/2015.phphttp://arhiva.nara.ac.rs/handle/123456789/10442022-06-16T08:56:39Z2022-06-16T08:56:39ZMicronucleus frequencies in peripheral blood lymphocytes in a Serbian human population exposed to pesticidesJovicic, DubravkaPajic, JelenaRadivojevic, LjiljanaRakic, BobanSaric-Krsmanovic, Marijahttp://arhiva.nara.ac.rs/handle/123456789/14312016-12-03T14:13:56ZMicronucleus frequencies in peripheral blood lymphocytes in a Serbian human population exposed to pesticides
Jovicic, Dubravka; Pajic, Jelena; Radivojevic, Ljiljana; Rakic, Boban; Saric-Krsmanovic, Marija
Micronucleus (MN) is a biomarker widely used in biomonitoring studies for determining
the genetic risk associated with exposure to pesticides. The purpose of this study was to
assess damage to the genetic material of workers occupationally exposed to pesticides as
detected in micronucleus tests. The research included 119 subjects divided into three groups:
a control group of 39 subjects, a group of 40 subjects exposed to pesticides as producers
and a group of 40 pesticide applicators in the field. A Mann-Whitney U-test displayed
statistically significant differences between the parameter means of all variables, and the
control group. Significant differences were observed between males involved in pesticide
production and application for the parameter MN4, then between non-smoking producers
and applicators regarding parameters MN2, MN3, MN4 and NB, as well as between the
control and applicator groups for parameter MN2, and between producers and applicators
for parameter MN3. Spearman’s correlation test showed a positive correlation between
the frequency of micronuclei and age of respondents, as well as their smoking habits.
A statistically significant difference in relation to cytogenetic parameters was detected
between the respondents working in pesticide production and those working in the field.
The results suggest that applicators in the field do not use adequate personal protective
equipment. Regular biological monitoring of workers exposed to pesticides is required.; Mikronukleus test (MN) se koristi u biomonitoring studijama za praćenje genetičkih efekata
kod ljudi izloženih pesticidima. Cilj rada je bio da se, metodom mikronukleus testa, utvrdi
oštećenje genetičkog materijala kod ispitanika koji su profesionalno izloženi pesticidima.
Istraživanja su obuhvatila 119 ispitanika koji su podeljeni u tri grupe: u kontrolnoj grupi bilo
je 39 ispitanika, u grupi izloženoj pesticidima u procesu proizvodnje (proizvođači) bilo je
40 ispitanika i 40 ispitanika koji rade u primeni na terenu. Mann-Whitney U-test je pokazao
statistički značajnu razliku između srednjih vrednosti parametara svih ispitivanih varijabli u
odnosu na kontrolnu grupu. Statistički značajne razlike konstatovane su između muškaraca u
proizvodnji i primeni za parametar MN4, potom između nepušača u proizvodnji i primeni za
parametere MN2, MN3, MN4 i NB, kao i u okviru cele posmatrane populacije između kontrolnih
i radnika u proizvodnji za parametar MN2, i između proizvodnje i primene za parametar MN3.
Spearman-ov test korelacije je pokazao pozitivnu korelaciju između učestalosti mikronukleusa
i starosne dobi ispitanika, kao i navika vezanih za pušenje. Utvrđene su i statistički značajne
razlike između ispitanika koji rade u proizvodnji i ispitanika koji rade u primeni u odnosu na
praćene citogenetičke parametre. Svi dobijeni rezultati upućuju na zaključak da radnici koji
rade na terenu ne koriste adekvatna sredstva lične zaštitite. Takođe, rezultati pokazuju da
postoji potreba za kontinuiranim biomonitoringom radnika koji su u kontaktu sa pesticidima.
Comparative characteristics of Lupinus albus L. and Lupinus luteus L. under allelopathic effect of Sorghum halepense L. (Pers.)Georgieva, NataliaNikolova, IvelinaMarinov-Serafimov, Plamenhttp://arhiva.nara.ac.rs/handle/123456789/14302016-12-03T14:14:07ZComparative characteristics of Lupinus albus L. and Lupinus luteus L. under allelopathic effect of Sorghum halepense L. (Pers.)
Georgieva, Natalia; Nikolova, Ivelina; Marinov-Serafimov, Plamen
Allelopathic effects of aqueous extracts of Sorghum halepense L. (Pers.) on seed
germination and primary seedling growth and development of two lupine species
was studied. Lupinus albus and Lupinus luteus showed different levels of susceptibility
to the allelopathic effect of weed extracts. Increasing concentrations (1.25, 2.50, 5.00
and 10.00%) of extracts from aboveground and belowground biomass suppressed
seed germination of L. luteus from 53.2 to 74.7%. The germination of L. albus seeds was
unaffected, except by the highest concentration of 10.00%. Fresh biomass accumulation in
the initial germ of L. luteus was inhibited by 3.8-40.3% under the effect of concentrations
of 2.50, 5.00 and 10.00%, which made the species susceptible to S. halepense extracts. L.
albus was tolerant as it was not found to sustain a significant allelopathic effect of the
extracts.; Proučavan je alelopatski uticaj vodenih rastvora Sorghum halepense L. (Pers.) na klijanje
semena i primarni rast i razvoj dve vrste lupina. Vrste Lupinus albus i Lupinus luteus su pokazale
različitu osetljivost na alelopatski uticaj rastvora ispitivanog korova. Rastuće koncentracije
(1.25, 2.50, 5.00 i 10.00%) ekstrakata nadzemne i podzemne biomase korova inhibirale
su klijanje semena L. luteus od 53.2 do 74.7%. Nije bilo uticaja na klijanje semena L. albus,
osim kod najviše koncentracije od 10.00%. Akumulacija biomase klice L. luteus inhibirana je
3.8-40.3% pod uticajem koncentracija od 2.50, 5.00 i 10.00%, što ovu vrstu čini osetljivom na
ekstrakt S. halepense. L. albus se pokazala kao otporna vrsta jer na nju ispitivani ekstrakti nisu
pokazali značajan alelopatski uticaj.
First record of spotted wing drosophila Drosophila suzukii (Diptera: Drosophilidae) in MontenegroRadonjic, SanjaHrncic, Snjezanahttp://arhiva.nara.ac.rs/handle/123456789/14292016-12-03T14:13:54ZFirst record of spotted wing drosophila Drosophila suzukii (Diptera: Drosophilidae) in Montenegro
Radonjic, Sanja; Hrncic, Snjezana
The spotted wing drosophila Drosophila suzukii Matsumura (Diptera: Drosophilidae)
is an invasive pest originating from Southeast Asia. It was detected for the first time in
Europe in 2008 (Spain and Italy) and subsequently in other European countries. It is a highly
polyphagous pest that infests healthy, ripening fruit and presents a serious threat to fruit
production, particularly of soft skinned fruit.
In the first half of October 2013, a new fruit fly species was unexpectedly detected in
Tephri traps baited with the three-component female-biased attractant BioLure that is
regularly used for monitoring the Mediterranean fruit fly Ceratitis capitata Wiedem. (Diptera:
Tephritidae) in Montenegro. Brief visual inspection identified the new species as the spotted
wing drosophila D. suzukii. The pest was first recorded in several localities on the Montenegrin
seacoast around Boka Kotor Bay. After the finding, all Drosophila specimens were collected
from traps for further laboratory observation. A quick follow-up monitoring of other Tephri
traps was carried out within the next few days on the rest of the seacoast (localities from
Tivat to Ulcinj). Additionally, Tephri traps were set up around Lake Skadar and in the city of
Podgorica, as well as on fresh fruit markets in Podgorica.
The results of this preliminary study showed that D. suzukii was present in all surveyed
locations and adults were captured until late December. Both sexes were found in traps
with BioLure.
Our data show that D. suzukii is present in southern parts of Montenegro and there is
a serious threat of its further spreading, particularly towards northern parts of the country
where the main raspberry and blueberry production is placed. The results also show that
Tephri traps baited with BioLure can be used for detection and monitoring of spotted wing
drosophila.; Drosophila suzukii (Diptera: Drosophilidae) je invazivna vrsta porijeklom iz jugoistočne Azije.
U Evropi je prvi put nađena 2008. godine (Španija i Italija), a nakon toga i u ostalim evropskim
zemljama. Ova veoma polifagna štetočina napada zdrave plodove u fazi zrenja i zbog toga
predstavlja veliku opasnost za voćarsku proizvodnju, a naročito za proizvodnju sitnog voća.
U prvoj polovini oktobra 2013. godine utvrđeno je prisustvo odraslih jedinki nove vrste
u Tephri klopkama sa trikomponentnim atraktantom BioLure koji se standardno koristi za
monitoring mediteranske voćne muve Ceratitis capitata Wiedem. (Diptera: Tephritidae) u
Crnoj Gori. Vizuelni pregled klopki ukazivao je da se radi o novoj vrsti, D. suzukii. Vrsta je
prvo zabilježena u lokalitetima u bokokotorskom zalivu (Baošići, Đenovići i Kumbor). Nakon
ovog nalaza sve jedinke Drosophila sp. su sakupljene radi daljeg laboratorijskog ispitivanja.
U narednih nekoliko dana izvršeni su pregledi Tephri klopki i na ostalom dijelu crnogorskog
primorja (lokaliteti od Tivta do Ulcinja). Pored toga, Tephri klopke su postavljene u okolini
Skadarskog jezera i na području Podgorice, uključujući i zelene pijace.
Rezultati ovog preliminarnog istraživanja su pokazali prisustvo D. suzukii u svim posmatranim
lokalitetima i hvatanje odraslih jedinki do kraja decembra. U klopkama sa BioLure nađene
su jedinke oba pola.
Utvrđeno prisustvo D. suzukii u južnom dijelu Crne Gore predstavlja veliku opasnost od
njenog daljeg širenja, posebno prema sjeveru zemlje koje je glavno proizvodno područje
maline i borovnice. Dobijeni rezultati, takođe, pokazuju da se Tephri klopke sa atraktantom
BioLure mogu koristiti za utvrđivanje prisustva i monitoring ove vrste.
In vitro sensitivity of Alternaria solani to conventional fungicides and a biofungicide based on tea tree essential oilStepanovic, MilosJevremovic, StojanRekanovic, EmilMihajlovic, MilicaMilijasevic-Marcic, SvetlanaPotocnik, IvanaTodorovic, Biljanahttp://arhiva.nara.ac.rs/handle/123456789/14282016-12-03T14:14:06ZIn vitro sensitivity of Alternaria solani to conventional fungicides and a biofungicide based on tea tree essential oil
Stepanovic, Milos; Jevremovic, Stojan; Rekanovic, Emil; Mihajlovic, Milica; Milijasevic-Marcic, Svetlana; Potocnik, Ivana; Todorovic, Biljana
A study of in vitro sensitivity of five Alternaria solani isolates to cooper-oxychloride,
chlorothalonil, difenoconazole, pyraclostrobin and a biofungicide based on tea tree
essential oil was carried out. The isolates were obtained from infected tomato leaves
collected from five different locations in Serbia. The tested isolates showed the highest
sensitivity to pyraclostrobin with EC50 values ranging from 0.0014 to 0.0041 μg ml-1. The
EC50 values of difenoconazole were 0.018-0.037 μg ml-1, chlorothalonil 2.99-4.54 μg ml-1, and
cooper-oxychloride 13.27-15.63 μg ml-1. All tested A. solani isolates were the least sensitive
to tea tree oil (1323.97-3307.08 μg l-1).; Ispitivana je osetljivost pet izolata Alternaria solani u in vitro uslovima na bakar-oksihlorid,
hlorotalonil, difenokonazol, piraklostrobin, kao i etarsko ulje čajnog drveta. Izolati su dobijeni iz
zaraženih listova paradajza sakupljenih sa pet lokaliteta na teritoriji Republike Srbije. Ispitivani
izolati su pokazali najveću osetljivost na piraklostrobin, sa EC50 vrednostima u intervalu od
0,0014 do 0,0041 μg/ml. Vrednosti EC50 za difenokonazol su bile od 0,018 do 0,037 μg/ml,
hlorotalonil 2,99-4,54 μg/ml i bakar-oksihlorid 13,27-15,63 μg/ml. Svi ispitivani izolati A. solani
su ispoljili najmanju osetljivost na etarsko ulje čajnog drveta (1323,97-3307,08 μg/ml).