Morphological and Pathogenic Characteristics of the Causal Agents of Dry and Wet Bubble Deseases of White Button Mushroom (Agaricus bisporus (Lange) Imbach) in Serbia

Abstract

Diseased fruit bodies of Agaricus bisporus, bearing symptoms similar to those caused by Verticillium fungicola and Mycogone perniciosa, were observed during the screening of eight mushroom farms in Serbia in 2002 and 2003. Nine isolates, forming either appressed white or pale brown colonies on PDA medium, were recovered. The isolates were identified on the basis of their morphological, physiological and pathogenic characteristics, and by comparing them to V. fungicola var. fungicola and M. perniciosa isolates originating from United Kingdom, and V. fungicola var. aleophilum from the USA. Pathogenicity of these isolates was confirmed by casing inoculation, on which occasion symptoms of both dry and wet bubble disease were recorded. Five isolates were identified as Verticillium fungicola (Preuss) Hassebrauk var. fungicola (W. Gams & Van Zaayen), and four as Mycogone perniciosa (Magnus) Delacroix.

Plodonosna tela Agaricus bisporus sa simptomima nalik onima koje izazivaju Verticillium fungicola i Mycogone perniciosa primećena su u osam gajilišta u Srbiji tokom 2002. i 2003. godine. Dobijeno je 9 izolata patogena koji obrazuju supstratne bele ili svetlo smeđe kolonije na PDA podlozi. Izolati su identifikovani na osnovu morfo-fizioloških karakteristika i patogenih osobina a determinacija potvrđena poređenjem sa kontrolnim izolatima V. fungicola var. fungicola i M. perniciosa iz Velike Britanije i V. fungicola var. aleophilum iz SAD. Veštačkim inokulacijama pokrivke za gajenje A. bisporus, dobijenim izolatima, pojavili su se simptomi suve i mokre truleži. Pet izolata je identifikovano kao Verticillium fungicola (Preuss) Hassebrauk var. fun­gi­co­la (W. Gams & Van Zaayen), a četiri kao Mycogone perniciosa (Magnus) Delacroix.