Genotoxicity of GAL-57 Herbicide in Salmonella typhimurium and Escherichia coli
Ispitivanja genotoksičnosti herbicida GAL-57 na Salmonella typhimurium i Escherichia coli
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Author
Brkic, Dragica
Gasic, Slavica
Vertesi, Adel
Karan, Vesela
Neskovic, Nesko
Keywords
GAL-57Mutagenicity
Bentazon
Dicamba
Ames test
mutagenost
bentazon
dikamba
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Show full item recordAbstract
Genotoxicity of the herbicide GAL-57, containing two active ingredients; bentazon and
dicamba, was investigated using the Ames test.
Salmonella typhimurium (tester strains TA98 and TA100) and Escherichia coli (strain
WP2uvrA) were used. Nine product concentrations were tested at a range of 19.53-5000 μg/plate and each concentration, as well as the controls, in triplicate. Testing was done with and without metabolic activation (liver microsomal fraction, S-9 mix). The results of our investigation revealed no biological or statistically significant increase in mutagenic factors, and this offered a basis for our conclusion that the herbicide GAL-57 has no genotoxic properties (with or without metabolic activation) under experimental conditions in the Ames test. Genotoksična svojstva herbicida GAL-57, koji u svom sastavu ima dve aktivne materije, bentazon i dikambu, ispitivana su primenom Ames-ovog testa. Korišćene su bakterije Salmonella
typhimurium (sojevi TA98 i TA100) i Escherichia
coli (WP2uvrA soj). Ispitivano je devet koncentracija preparata u opsegu 19.53-5000 μg/ploča pri čemu je svaka koncentracija, kao i kontrole, testirana po tri puta. Ispitivanje je obavljeno sa i bez metaboličke aktivacije
(mikrozomska frakcija jetre, S-9 mix). Rezultati
ispitivanja su pokazali da nema ni biološki ni statistički značajnog povećanja faktora mutacije
na osnovu čega se zaključuje da herbicid
GAL-57, u eksperimentalnim uslovima Ames-ovog testa, ne poseduje genotoksična svojstva (sa i bez metaboličke aktivacije).