Methods of Imazethapyr Extraction from Soil and Cleaning up by Solid Phase Extraction

Abstract

The aim of this work was to optimize the determination methods of imazethapyr residues in soil using HPLC with DAD and C 18 (Hypersil ODS, 5 ìm, 2.0 x 250 mm) columns and to find the most appropiate solvent for imazethapyr extraction as well as the most suitable SPE cartridges for extract purification. For soil extract purification we decided to use SPE Amino/Carbon, C 18 and SDB-1 cartridges. On the basis of the perfomed chromatography method for optimising imazethapyr determination in soil it was found that the most appropiate method for determining imazethapyr residues in soil is the extraction method with Na2CO3 solution, followed by cleaning up on SPE SDB-1 catridge and elution with ACN and water.

Ovim istraživanjem je obuhvaćeno iznalaženje optimalnih eksperimentalnih uslova za određivanje ostataka imazetapira u zemljištu, korišćenjem tečne hromatografije sa DAD detektorom i uz primenu C18 (Hypersil ODS, 5 μm, 2.0 x 250 mm) kolone, uz iznalaženje najpogodnijeg rastvarača za ekstrakciju i najpogodnije kolone za ekstrakciju u čvrstoj fazi (SPE) za prečišćavanje dobijenog ekstrakta. Pri prečišćavanju ekstrakta zemlje korišćene su aminokarbon, C18 i SDB-1 SPE kolone. Na osnovu izvršene optimizacije hromatografske metode određivanja ostataka imazetapira u zemljištu utvrđeno je da je najpogodniji postupak metoda ekstrakcije rastvorom Na2CO3, prečišćavanjem preko SPE SDB-1 kolone i eluiranje mešavinom acetonitrila (ACN) i vode (75:25 v/v).