Effects of Metribuzin on the Activity of Some Enzymes in Soil

Abstract

Eeffects of metribuzin on the activity of some enzymes in soil was investigated. Trials were set up in the laboratory on a clay loam soil. Metribuzin was applied at 12.0, 24.0 and 60.0 mg/kg soil rates and soil samples were collected 3, 7, 15, 30 and 45 days after metribuzin treatment for analyses. Alkaline phosphatase, acid phosphatase, dehydrogenase, urease and β-glucosidase were recorded. The results showed that the intensity of metribuzin effects on the activity of enzymes depended on treatment rate, exposure time and enzyme group. Metribuzin had an inhibiting effect on acid phosphatese and dehydrogenase, as well as on alkaline phosphatase in the initial stage before it turned into a stimulating one, while metribuzin stimulated urease and had no effect on β-glucosidase.

U radu je ispitivano delovanje metribuzina na aktivnost nekih enzima u zemljištu. Ogled je postavljen u laboratorijskim uslovima na zemljištu tipa černozem. Metribuzin je primenjen u količinama 12,0, 24,0 i 60,0 mg/kg zemljišta, a uzorci za analizu uzimani su 3, 7, 15, 30 i 45 dana posle primene metribuzina. Praćena je aktivnost enzima alkalna i kisela fosfataza, dehidrogenaza, ureaza i β-glukozidaza. Dobijeni rezultati su pokazali da delovanje metribuzina na aktivnost enzima zavisi od primenjene količine, dužine delovanja i vrste enzima. Metribuzin je delovao inhibitorno na kiselu fosfatazu i dehidrogenazu, na alkalnu fosfatazu prvo je delovao inhibitorno, a kasnije stimulativno, na ureazu je delovao stimulativno, dok na β-glukozidazu nije delovao.