Effects of Two Formulations Based on Bacillus thuringiensis subsp. israelensis on Aedes aegypti Mosquito Larvae under Laboratory Conditions
Efekti dve formulacije na bazi Bacillus thuringiensis subsp. israelensis na larve komarca Aedes aegypti u laboratorijskim uslovima
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Author
Peric, Milenka
Prijovic, Mirjana
Andric, Goran
Keywords
Bacillus thuringiensis subsp. israelensisVectoBac WDG
VectoBac 12AS
Aedes aegypti larvae
Persistance
trajnost delovanja
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Show full item recordAbstract
Toxicity and persistence of two formulations based on Bacillus thuringiensis subsp. israelensis
applied to Aedes aegypti mosquito larvae were tested under laborabory conditions.
The formulations were: a) water dispersible granules (product VectoBac WDG), and b) aqueous suspension (product VectoBac 12AS). The effects of both products on mosquito larvae
were tested immediately after their dilution in pure water (zero time) and in 1-, 2-, 8- and
13-day old solutions. The two products were also tested in mixtures of water and clay at a
rate of 0.5 g clay/150 ml water immediately after product dilution, and in one-day old solutions
containing 0.1 and 0.05 g of clay in the same amount of water. The product VectoBac
WDG was persistant and highly effective against Ae. aegypti larvae in pure water after the
longest experimental period of 13 days, and significantly more effective than VectoBac
12AS at equal rates of application. The effectiveness of VectoBac 12AS weakened significantly
after 8 and 13 days of treatment, compared to the effects at zero time and in 1- and
2-day old solutions. High clay content in water significantly reduced the larvicidal effectiveness
of both products, indicating its possible compromising role during product application
in practice. U laboratorijskim uslovima ispitivana je toksičnost i trajnost delovanja na larve komarca
Aedes aegypti dve formulacije na bazi Bacillus thuringiensis subsp. israelensis: a) vododisperzibilne
granule (preparat VectoBac WDG) i b) tečna suspenzija (preparat VectoBac 12AS).
Utvrđivani su efekti oba preparata na larve u čistoj vodi neposredno posle njihovog rastvaranja
(nulto vreme), a potom u rastvorima starim 1, 2, 8 i 13 dana. Takođe, testirani su efekti
preparata u vodi sa glinom u količini 0,5 g na 150 ml vode, neposredno posle pravljenja
rastvora, i efekti preparata u istoj zapremini vode sa količinama gline od 0,1 i 0,05 g, u rastvorima
starim jedan dan. Konstatovano je da je preparat VectoBac WDG perzistentan i visoko
efektivan u delovanju na larve Ae. aegypti u čistoj vodi tokom 13 dana posle primene
(koliko je trajao eksperiment) i u istim dozama značajno je efektivniji nego VectoBac 12AS.
Efektivnost preparata VectoBac 12AS značajno slabi osam i 13 dana nakon primene u poređenju
sa efektima ispoljenim u nulto vreme i jedan i dva dana kasnije. Kod oba preparata
larvicidnu aktivnost značajno smanjuje visok sadržaj gline u vodi, što ukazuje na njenu moguću
ulogu u kompromitovanju praktične primene ovih preparata.