Biological Variability of Zucchini yellow mosaic virus in Serbia
Biološka varijabilnost virusa žutog mozaika cukinija u Srbiji
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Author
Vucurovic, Ana
Bulajic, Aleksandra
Djekic, Ivana
Ristic, Danijela
Berenji, Janos
Krstic, Branka
Keywords
PumpkinZucchini yellow mosaic virus
Biological characterization
Primer suitability
Tikve
virus žutog mozaika cukinija
biološka karakterizacija
pogodnost prajmera
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Show full item recordAbstract
Zucchini yellow mosaic virus (ZYMV) has become an important pathogen of cucurbits
over the past 20 years. In our country, this virus also causes significant losses, especially in
oil pumpkin production. Although ZYMV variability has been extensively studied worldwide
by biological and molecular characterization, such information is still limited for the
European region. Herein, we present the first analysis of phenotypic variations of ZYMV isolates
sampled from oil pumpkin cv. Olinka from different locations in Serbia during 2008.
Twelve isolates were obtained by mechanical inoculation on the C. pepo hybrid Ezra F1, and
four of them were chosen for further detailed biological study. These investigated isolates
exhibited different phenotypical features and thus represented different variants, concerning
their host range, and type and severity of symptoms. A previously developed primer
pair, ZYMV CPfwd/CPrev, was tested to determine its suitability for detection of Serbian
ZYMV isolates. Amplification of Serbian ZYMV isolates of different origin, in single and
mixed infection, resulted in one distinctive band. The used primer pair proved to be useful
not only for routine molecular detection, but also for further molecular characterization of
Serbian ZYMV isolates because it amplifies the hypervariable genomic region of ZYMV.
The result obtained in this study is a contribution to investigation of genetic diversity
of Serbian ZYMV isolates and their molecular relationship with ZYMV isolates from
other parts of the world. Poslednjih 20 godina virus žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV)
se javlja kao značajan patogen biljaka iz familije tikava. Kod nas ovaj virus, takođe, izaziva
značajne gubitke, naročito u proizvodnji uljane tikve. Iako je biološka i molekularna varijabilnost
ZYMV dosta ispitivana, još uvek nema dovoljno informacija o varijabilnosti evropskih
izolata ovog virusa. Ovaj rad predstavlja prvu analizu fenotipske varijabilnosti izolata ZYMV
poreklom iz Srbije, koji su tokom 2008. godine sakupljeni iz različitih lokaliteta gajenja uljane
tikve sorte Olinka. Mehaničkim inokulacijama C. pepo hibrid Ezra F1, dobijeno je 12 izolata,
od kojih su četiri odabrana za dalju biološku karakterizaciju. Na osnovu različitog kruga
domaćina, tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih
izolata. Par prajmera ZYMV CPfwd/Cprev testiran je radi utvrđivanja pogodnosti za
rutinsku detekciju izolata ZYMV poreklom iz Srbije. Na osnovu pojave traka očekivane veličine
u gelu, ZYMV je detektovan i u pojedinačnim i mešanim infekcijama u biljkama sa različitih
lokaliteta, što dokazuje pogodnost ovih prajmera. Kako ovi prajmeri amplifikuju hipervarijabilni
deo genoma virusa, pogodni su ne samo za rutinsku molekularnu detekciju, već
i za molekularnu karakterizaciju izolata ZYMV iz Srbije.
Rezultati dobijeni u ovom radu omogućiće dalja proučavanja genetičke varijabilnosti
izolata ZYMV iz Srbije, kao i njihove evolutivne povezanosti sa izolatima iz drugih delova
sveta.