Morphological, Serological and Molecular Analyses of Anthracnose-Causing Agent on Banana Fruit
Morfološke, serološke i molekularne analize prouzrokovača antraknoze ploda banane
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Author
Duduk, Natasa
Ivanovic, Mirko
Duduk, Bojan
Keywords
BananaAnthracnose
Colletotrichum gloeosporoides
Colletotrichum musae
Identification
Banana
antraknoza
identifikacija
Metadata
Show full item recordAbstract
Two species of the genus Colletotrichum, C. musae and C. gloeosporoides, occur as infecting
species of banana. The study focused on examining the etiology of anthracnose on
banana fruits sold on the domestic market. An isolate was obtained from a deseased banana
fruit on PDA medium, forming a white colony with intensive and uniformed growth. It was
not possible to identify the isolated fungus based on its morphological characteristics.
Positive serological reaction in an ELISA test with monoclonal antibodies for C. acutatum
indicated an antigen site for the used monoclonal antibodies. Positive reaction when C.
gloeosporioides-specific primers were applied indicated a similarity in the ITS sequence of
the fungus and the examined isolate from banana fruit. Although there are no available
data in literature that C. gloeosporioides-specific CgInt primer can be used for amplification
of the phylogenetically related C. musae, our results do not exclude that the isolate could be
C. musae. The host plant, symptoms observed and colony characteristics of the fungus isolated
from the banana fruit mostly correspond to C. musae. Based on morphological, antigen
and gentic characteristics, the isolate from banana was determined as Colletotrichum
sp., while species identification of the anthracnose-causing agent on banana requires additional
analysis. Na banani se javljaju dve vrste iz roda Colletotrichum, C. musae i C. gloeosporoides. Cilj
ovog rada bio je ispitivanje etiologije antraknoznog propadanja plodova banane koje se u
našoj zemlji nalaze u prometu. Iz obolelog ploda banane dobijen je izolat koji na PDA podlozi
formira koloniju bele boje sa intenzivnim uniformnim rastom. Na osnovu dobijenih
morfoloških osobina izolovane gljive nije se mogla definitivno uraditi identifikacija. Pozitivna
serološka reakcija u ELISA testu sa monoklonalnim antitelima za C. acutatum govori o postojanju
antigenog mesta za korišćena monoklonalna antitela. Pozitivna reakcija korišćenjem
prajmera specifičnih za C. gloeosporioides ukazuje na sličnost u sekvenci ITS regiona ove
gljive i ispitivanog izolata iz banane. Mada u literaturi nije zabeleženo da se korišćenjem
CgInt prajmera specifičnog za C. gloeosporioides može amplifikovati i njemu filogenetski
bliska C. musae, ovi rezultati ne isključuju mogućnost da je izolat iz banane C. musae. Biljka
domaćin, zabeleženi simptomi i osobine kolonije izolata iz banane najviše odgovaraju C.
musae. Na osnovu dobijenih rezultata morfoloških, antigenih i genetskih osobina izolat iz
banane označen je kao Colletotrichum sp., a za tačnu identifikaciju prouzrokovača antraknoze
ploda banane do nivoa vrste neophodno je sprovesti dalje analize.