Raspberry bushy dwarf virus – a Grapevine Pathogen in Serbia
Virus žbunaste kržljavosti maline (Raspberry bushy dwarf virus) – patogen vinove loze u Srbiji
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Author
Jevremovic, Darko
Paunovic, Svetlana
Keywords
Raspberry bushy dwarf virusGrapevine
Detection
DAS-ELISA
Nested-PCR
Virus žbunaste kržljavosti maline
vinova loza
detekcija
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Show full item recordAbstract
During a field survey in 2005 in vineyards and grapevine nurseries at localities in
central Serbia, a few plants with unusual virus-like symptoms were observed. Leaf samples
were analyzed by DAS-ELISA for the presence of nine viruses. Besides other viruses
frequently occurring on grapevine, Raspberry bushy dwarf virus (RBDV) was detected in
two samples. Results were confirmed by nested-PCR and sequence analysis of the fragment
in 5’ part of RNA-1. Obtained sequence shared at least 93% of nucleotide identity
with the compared RBDV sequence originating from raspberry. The finding of Raspberry
bushy dwarf virus on grapevine in Serbia is a second finding of this pathogen on grapevine
worldwide. The first natural infection of grapevine with this virus was reported in
Slovenia in 2003. Pregledom vinograda i rastila vinove loze tokom 2005. godine u lokalitetima centralne
Srbije na nekoliko biljaka su primećeni neuobičajeni simptomi koji su ukazivali na moguće
prisustvo virusa. Uzorci listova su analizirani DAS-ELISA testom na prisustvo 9 virusa. Pored
virusa koji se često javljaju na vinovoj lozi, kod dva uzorka je potvrđeno prisustvo virusa žbunaste
kržljavosti maline (Raspberry bushy dwarf virus, RBDV). Nested-PCR metodom i sekvencionom
analizom fragmenta iz 5’ regiona RNA-1 segmenta genoma RBDV potvrđeni su rezultati
serološkog testa. Dobijena nukleotidna sekvenca pokazuje najmanje 93% sličnosti sa
upoređenom sekvencom RBDV izolata iz maline. Potvrda prisustva RBDV na vinovoj lozi u
Srbiji je drugi nalaz ovog patogena na vinovoj lozi u svetu. Prvi nalaz prirodne infekcije vinove
loze ovim virusom saopšten je u Sloveniji 2003. godine.