Fusarium oxysporum as Causal Agent of Tomato Wilt and Fruit Rot
Identifikacija prouzrokovača uvenuća i truleži plodova paradajza
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Author
Ignjatov, Maja
Milosevic, Dragana
Nikolic, Zorica
Gvozdanovic-Varga, Jelica
Jovicic, Dusica
Zdjelar, Gordana
Keywords
WiltsTomatoes
Nutrients
Isolation
Fusarium
Fruits
Uvenuće
paradajz
hraniva
izolacija
voće
Metadata
Show full item recordAbstract
Tomatoes are parasitized by a number of pathogens, including Fusarium oxysporum f. sp.
lycopersici, causal agent of fusarium wilt. Fresh vegetable fruits can be contaminated with various
fungi that produce mycotoxins, which is an important issue for human health. The objective
of this paper was to isolate, determine, and identify causal organisms of tomato wilt and
fruit rot, based on the pathogens morphological and molecular characteristics. Samples of
diseased plants showing symptoms of tomato wilt were collected from different localities in
the production region of Vojvodina. Fruits with symptoms of fusarium rot were collected from
storage and warehouses. The isolation and morphological determination of the fungus were
performed on PDA and Czapek’s nutrient media. Isolates from diseased plants growing in field,
designated as TFW1-TFW12 and seven isolates from diseased tomato fruits (TFM1-TFM7) were
chosen for further investigation. For identification of the fungal isolates, the polymerase chain
reaction (PCR) was also used. The EF1/EF2 primer pair was used for molecular identification
of Fusarium sp. Nine analyzed samples were found to contain DNA fragments 700 bp in size. Patogene gljive roda Fusarium, prouzrokovači uvenuća biljaka, pojavljuju se i kao paraziti
povrća uzrokujući propadanje korena, prizemnog dela stabla, kao i uvenuće biljaka. Veći broj
patogena parazitira paradajz, među kojima je i Fusarium oxysporum f. sp. Lycopersici, prouzrokovač
fuzarioznog uvenuća. Fuzariozna trulež plodova paradajza redovno se javlja u skladištima
i može naneti velike ekonomske gubitke. Cilj ovog rada bio je izolacija, utvrđivanje i identifikacija
prouzrokovača uvenuća paradajza i truleži plodova, na osnovu morfoloških i molekularnih
karakteristika patogena. Prikupljeni su uzorci obolelih biljaka sa simptomima uvenuća paradajza
iz proizvodnih regiona Vojvodine. Sveži plodovi sa simptomima fuzariozne truleži prikupljeni
su iz različitih magacina i skladišta. Za izolaciju i morfološku determinaciju gljive korišćene
su PDA i Czapek hranljiva podloga. Za dalja proučavanja odabrano je 12 izolata (TFW1-TFW12)
poreklom sa biljaka gajenih u polju i sedam izolata (TFM1-TFM7) dobijenih sa zaraženih plodova
iz skladišta. Determinacija izolata gljiva obavljena je i pomoću metode, lančane reakcije polimeraze
(PCR – Polymerase Chain Reaction). Za molekularnu identifikaciju izolata F. oxysporum
korišćen je par prajmera EF1/EF2. U devet ispitivanih uzoraka potvrđeno je prisustvo DNK fragmenta
veličine 700 bp.