Botrytis cinerea in raspberry in Serbia II: Growth rate and virulence of isolates

Abstract

Growth rate and virulence of 130 isolates of Botrytis cinerea, derived from raspberry fruit originating from six commercial fields in a raspberry growing region of Serbia and classified in two morphological and four genetic groups were studied. The results showed significant differences in mycelial growth rate among the isolates. The highest and lowest recorded growth rates were 24.5 mm/day and 8.4 mm/day, respectively, while the growth rate of most isolates ranged from 15.8 to 21.8 mm/day. The growth rate of isolates that belong to different morphological and genetic subgroups varied similarly. Furthermore, growth rate intervals of all subgroups overlapped, suggesting that the groups cannot be distinguished based on growth rates of the isolates contained. The studied B. cinerea isolates exibited different levels of virulence towards vine, sunflower and raspberry leaves, while an analysis of variance revealed that both the isolates and the inoculated host species were significant sources of variation (P<0.01). Sunflower and raspberry leaves were significantly more sensitive than vine leaves. However, correlation between isolate virulence and different hosts was not found.

U radu su predstavljeni rezultati proučavanja brzine rasta i virulentnosti 130 izolata Botrytis cinerea, dobijenih iz obolelih plodova maline poreklom sa šest lokaliteta iz područja komercijalnog gajenja maline u Srbiji i razvrstanih u dve morfološke i četiri genetičke grupe. Rezultati su pokazali da je razlika između izolata u brzini rasta statistički značajna. Najveći zabeleženi porast bio je 24,5 mm/dan, dok je najmanji porast iznosio 8,4 mm/dan. Utvrđena su slična variranja u porastu izolata koji pripadaju različitim morfološkim ili genetičkim grupama. Drugim rečima, rasponi brzine rasta izolata iz različitih morfoloških i genetičkih grupa međusobno se preklapaju, što ukazuje da ovaj parametar nije pogodan za razvrstavanje izolata u grupe. Proučavani izolati su ispoljili različit nivo virulentnosti za listove vinove loze, suncokreta i maline, dok je analiza varijanse pokazala da su i izolati i domaćini statistički značajan izvor variranja (P<0,01). Listovi suncokreta i maline bili su značajno osetljiviji od listova vinove loze. Međutim, korelacija u virulentnosti izolata za različite domaćine nije ustanovljena.