Botrytis cinerea in raspberry in Serbia I: Morphological and molecular characterization
Botrytis cinerea na malini u Srbiji I: Morfološka i molekularna karakterizacija
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Author
Tanovic, Brankica
Hrustic, Jovana
Mihajlovic, Milica
Grahovac, Mila
Delibasic, Goran
Keywords
Botrytis cinereaRaspberries
Serbia
malina
Srbija
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Show full item recordAbstract
Morphological and molecular characterisation of 130 isolates of Botrytis cinerea, derived
from raspberry fruit originating from six commercial fields in a raspberry growing region
of Serbia (locations: Požega, Prilike, Arilje, Ivanjica, Šabac and Valjevo) was performed. The
results showed that all isolates formed white, uniform, aerial mycelia with entire margin on
PDA medium. First morphological differences among the isolates appeared after six days
of incubation. Three-week old isolates were grouped into eight distinct morphological
types – four mycelial and four sclerotial. Mostly, they were of sclerotial type (81.5%) and
the most frequently found was an S3 type, which formed large irregularly placed sclerotia.
This type was dominant in five of six investigated locations and represented 45-65% of the
isolates. The least frequent was the mycelial type M3 (0.7% of the isolates) characterized
by mycelial masses.
The presence of Boty and/or Flipper transposons was detected in isolates originating
from all investigated locations. It was discovered that the B. cinerea population in raspberry
in Serbia, besides the well-described genetically isolated sympatric species transposa
(43.1%) and vacuma (10.8%), contains also another two, boty (44.6%) and flipper (1.5%)
species with only one transposon (either Boty or Flipper) in the genome. In addition, it
was revealed that all isolates from raspberry collected in Serbia, transposa, vacuma, boty
or flipper, are sensitive or weakly resistant to fenhexamid and therefore belong to the B.
cinerea genetical Group II. U radu su predstavljeni rezultati morfološke i molekularne karakterizacije 130 izolata
Botrytis cinerea, dobijenih iz obolelih plodova maline poreklom sa šest lokaliteta iz područja
komercijalnog gajenja maline u Srbiji (Požega, Prilike, Arilje, Ivanjica, Šabac i Valjevo). Utvrđeno
je da u početnim fazama razvoja na KDA podlozi svi izolati B. cinerea formiraju belu, uniformnu,
rastresitu, vazdušnu miceliju ravnog oboda. Razlike među izolatima počinju da se javljaju
posle inkubacije od šest dana. Na osnovu izgleda kolonije tri nedelje od zasejavanja, izolati
su razvrstani u osam morfoloških tipova – četiri micelijska i četiri sklerocijska. Većina izolata
je formirala kolonije sklerocijskog tipa (81,5%), a najzastupljeniji je bio tip S3 sa krupnim,
nepravilno raspoređenim sklerocijama, koji je dominirao u pet od šest proučavanih populacija
patogena i predstavljao 45-65% izolata. Najređi je bio micelijski tip M3 (0,7% izolata) koji se
odlikuje nakupinama vazdušne micelije.
Prisustvo transpozona Boty i/ili Flipper otkriveno je u genomu izolata sa svih lokaliteta.
Utvrđeno je da u populaciji patogena na malini u Srbiji, osim genetički izolovanih subpopulacija
transposa (43,1%) i vacuma (10,8%), postoje još dve – boty (44,6%) i flipper (1,5%) sa izolatima
koji sadrže samo jednu vrstu transpozona u genomu. Istraživanje je takođe pokazalo da su
svi izolati B. cinerea na malini u Srbiji, bilo da su transposa, vacuma, boty ili flipper, osetljivi ili
slabo rezistentni na fenheksamid i da, prema tome, pripadaju genetičkoj Grupi II B. cinerea.