Pectobacterium carotovorum subsp. carotovorum – the causal agent of broccoli soft rot in Serbia
Pectobacterium carotovorum subsp. carotovorum prouzrokovač vlažne truleži brokolija u Srbiji
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Author
Gasic, Katarina
Gavrilovic, Veljko
Dolovac, Nenad
Trkulja, Nenad
Zivkovic, Svetlana
Ristic, Danijela
Obradovic, Aleksa
Keywords
BroccoliPectobacterium carotovorum subsp. carotovorum
Soft rot
16S rRNA gene
ITS-PCR-RFLP
Brokoli
vlažna trulež
Metadata
Show full item recordAbstract
Soft rot symptoms were observed on broccoli plants in several commercial fields in the
western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as
Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular
methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative
and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive
reaction on tobacco leaves. They grew in 5% NaCl and at 37°C, did not produce acid from á-methyl
glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and
trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic
growth on Logan’s medium and did not produce blue pigmented indigoidine on GYCA medium
nor “fried egg” colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP
analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused
tissue discoloration and soft rot development on inoculated broccoli head tissue fragments. Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj
Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium
carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih
molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni,
fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući
vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali
hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37ºC i u prisustvu 5% NaCl, ne
proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz
saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su
karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment
na GYCA podlozi niti “fried egg” kolonije na PDA podlozi. Identifikacija sojeva potvrđena je
korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja
testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih
drški brokolija.