Effect of Nitrogen Forms and Concentrations on Growth and the Pigments Content of Ocimum Basilicum L. Cultured In Vitro

Abstract

The aim of this study was to examine the effect of nitrogen on the growth and concentration of photosynthetic pigments of Ocimum basilicum L. cultured in vitro. Six media were used for research, which differed in form and concentration of nitrogen. According to the obtained results, a significant effect on the roots and shoots length, as well as the concentration of chlorophyll and carotenoids had a nitrate form of nitrogen in contrast to the combination of nitric and ammonium forms. Based on obtained results, it is recommended cultivation basil plants inmedia withpotassium nitrate.

Cilj istraživanja obuhvata utvrđivanje uticaja azota na rast i koncentraciju fotosintetičkih pigmenata bosiljaka (Ocimum basilicum L.) proizvedenog u kulturi in vitro. Za istraživanjeje upotrebljenošesthranljivih podloga, koje su se razlikovale po formi i koncentraciji azota. Prema dobijenim rezultatima, značajniji efekat na rast i koncentraciju hlorofila i karotenoida imala je nitratna forma azota u odnosu na kombinaciju nitratne i amonijačne forme. Utvrđeno je da je u procesu gajenja bosiljka kalijum-nitrat pogodan za oplemenjavanje zemljišta.