In Vitro Propagation of Contemporary Vegetative Rootstocks for Plum

Abstract

To determine the effect of successive subculturing on multiplication capacity of shoots, vegetative rootstocks for plum Docera, Dospina and St. Julien A were repeatedly subcultured for 5 subcultures on medium of unchanged composition. As for Docera and Dospina, significan increase in multiplication index and length of axial shoots was observed in the 3. subculture, whereupon their values gradually decreased to the 5. subculture, but remained considerabely higher in comparison with the values in the first two subcultures. Contrary, in St. Julien A shoot multiplication declined over the first three subcultures, then increased and remained at the level of the 1. subculture. Length of axial shoots was changed in the same way as for Docera and Dospina. The highest cacpacity for in vitro rooting was observed in Docera (91.7%), while the lowest was in St. Julien A (44.4%).

Vegetativne podloge za šljivu Docera, Dospina i St. Julien A su umnožavane in vitro pet uzastopnih supkultura na medijumu konstantnog sastava u cilju ispitivanja uticaja sukcesivnog supkultivisanja na parametre multiplikacije. Kod podloga Docera i Dospina uočeno je značajno povećanje indeksa multiplikacije i dužine osovinskih izdanaka u 3. supkulturi, posle čega njihova vrednost postepeno opada do 5. supkulture, ali i dalje ostaje značajno veća u odnosu na prve dve supkulture. Suprotno, kod podloge St. Julien A uočen je trend pada indeksa multiplikacije od 1. ka 3. supkulturi, posle čega njegova vrednost ponovo raste i ostaje na nivou 1. supkulture. Dužina osovinskih izdanaka se tokom supkultivisanja menjala na isti način kao kod podloga Docera i Dospina. Najveći kapacitet ožiljavanja in vitro utvrđen je kod podloge Docera (91,7%), a najniži kod St. Julien A (44,4%).