Laboratory Tests for Group and Individual Exposures of Arion lusitanicus Mabille Slugs to Different Molluscicide Baits
Laboratorijski testovi grupnog i individualnog izlaganja jedinki vrste Arion lusitanicus Mabille različitim moluskocidnim mamcima
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Author
Stojnic, Bojan
Vuksa, Marina
Jokic, Goran
Keywords
Slug Arion lusitanicus MabilleMolluscicides
Fe pyriphosphate hydrate
Laboratory tests
Puž Arion lusitanicus Mabille
moluskocidi
Fe pirofosfat hidrat
laboratorijski testovi
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Show full item recordAbstract
Molluscicide baits based on different active ingredients were tested in a seven-day laboratory
trial on juveniles and young adults of Arion lusitanicus Mabille slug collected in ruderal
sites during June and July of 2008. Before setting the trial, the slugs were adapted to
laboratory conditions.
The testing was conducted using a modified version of the method proposed by
Godan (1983) and Wiktor (1989). The slugs were kept in arenas under controlled conditions
(20-24oC temperature, 80-90% relative air humidity, no air stream, diffuse daylight). Smaller
(15.5 x 15.5 x 7 cm) and larger (28 x 17.5 x 7 cm) arenas were used, depending on slug numbers,
and different rates of food and bait were administered. Plastic boxes with perforated
coverage, lined with multi-ply paper moistened on a daily basis, were used as arenas. The
slugs were fed on fresh salad daily, while baits were administered in open 35 mm petri dishes
once for the duration of experiment. The first trial involved single-slug exposures to bait
in 20 arenas per each of four treatments with two replicates and a total of 160 slugs. The
second trial, group exposure, involved four treatments of five slugs per arena in six replicates
with a total of 120 slugs.
The products Arion and Pužomor demonstrated the highest efficacy (77.5%) in the single-
slug trial. The average efficacy of the product Carakol after seven days of exposure was
60.0%. Regarding group exposure, Pužomor pellets achieved 79.3% efficacy after three
days and this efficacy rate remained for the rest of the experiment. On the seventh day of
exposure, Arion and Carakol had 33.3% and 40.0% efficacy, respectively. Sedmodnevno laboratorijsko testiranje delovanja moluskocidnih mamaka na bazi različitih
aktivnih materija obavljeno je na juvenilima i mladim adultima golaća Arion lusitanicus
Mabille, prikupljenim na ruderalnim površinama tokom juna i jula meseca 2008. godine. Pre
postavljanja ogleda, prikupljeni golaći adaptirani su na laboratorijske uslove.
Testiranje je izvedeno prema prilagođenoj metodi koju su predložili Godan (1983) i
Wiktor (1989). Puževi su držani u arenama, u kontrolisanim uslovima (temperatura 20-24oC,
relativna vlažnost 80-90%, bez strujanja vazduha i uz difuzno dnevno svetlo). Zavisno od
broja jedinki, primenjene su manje (15,5 x 15,5 x 7 cm) i veće (28 x 17,5 x 7 cm) arene i različite
količine hrane i mamaka. Kao arene su upotrebljene plastične kutije sa perforiranim poklopcem,
obložene višeslojnom hartijom, koja je vlažena jednom dnevno. Puževi su dnevno
hranjeni svežom salatom, a mamak je postavljan u otvorene petrijeve posude prečnika
35 mm, i nije menjan tokom eksperimenta. Prvi ogled, individualno izlaganje golaća mamcima,
obavljen je sa četiri tretmana po 20 arena, u dva ponavljanja, sa ukupno 160 puževa.
Drugi ogled, grupno izlaganje mamcima, obavljen je sa četiri tretmana po pet puževa u areni,
u šest ponavljanja, sa ukupno 120 puževa.
U eksperimentu sa jednim pužem u areni, najveću efikasnost postigli su preparati Arion
i Pužomor pelete (77,5%). Prosečna efikasnost preparata Carakol, posle sedam dana ekspozicije,
bila je 60,0%. Grupnim izlaganjem puževa mamcima, preparat Pužomor pelete, trećeg
dana ekspozicije, pokazao je efikasnost od 79,3%, koja se nije menjala do kraja eksperimenta.
Sedmog dana ekspozicije efikasnost preparata Arion i Carakol bila je 33,3%, odnosno
40,0%.