Naturally-Occurring Entomopathogenic Fungi on Three Bark Beetle Species (Coleoptera: Curculionidae) in Bulgaria
Entomopatogene gljive koje se u prirodi javljaju kod tri vrste potkornjaka (Coleoptera: Curculionidae) u Bugarskoj
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Author
Draganova, Slavimira
Takov, Danail
Doychev, Danail
Keywords
Bark beetlesIps sexdentatus
Ips typographus
Dryocoetes autographus
Entomopathogenic fungi
Beauveria bassiana
Beauveria brongniartii
Isaria farinosa
Morphological characteristics
Natural occurrence
Potkornjaci
entomopatogene gljive
morfološke osobine
pojava u prirodi
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Show full item recordAbstract
Bark beetles (Coleoptera: Curculionidae: Scolytinae) belong to one of the most damaging
groups of forest insects and the activity of their natural enemies – pathogens, parasitoids,
parasites or predators suppressing their population density, is of great importance.
Biodiversity of entomopathogenic fungi on bark beetles in Bulgaria has been investigated
sporadically. The aim of this preliminary study was to find, identify and study morphological
characteristics of fungal entomopathogens naturally-occurring in populations
of three curculionid species – Ips sexdentatus Boern, Ips typographus (L.) and Dryocoetes
autographus (Ratz.).
Dead pest adults were found under the bark of Pinus sylvestris and Picea abies trees collected
from forests in the Maleshevska and Vitosha Mountains. Fungal pathogens were
isolated into pure cultures on SDAY (Sabouraud dextrose agar with yeast extract) and
were identified based on morphological characteristics both on the host and in a culture.
Morphological characteristics of the isolates were studied by phenotypic methods.
The fungal isolates obtained from dead adults of Ips sexdentatus, Ips typographus and D.
autographus were found to belong to the species Beauveria bassiana (Bals. – Criv.) Vuillemin,
Beauveria brongniartii (Saccardo) Petch and Isaria farinosa (Holmsk.) Fries (anamorph
Ascomycota, Sordariomycetes: Hypocreales, Cordycipitaceae). Morphological traits of the
isolates are described. Potkornjaci (Coleoptera: Curculionidae: Scolytinae) pripadaju jednoj od najštetnijih grupa
šumskih insekata, te je delovanje njihovih prirodnih neprijatelja, patogena, parazitoida,
parazita i predatora od velikog značaja.
Biodiverzitet entomopatogenih gljiva koje se javljaju kod potkornjaka u Bugarskoj malo
je istraživan. Cilj ovog početnog ispitivanja bio je da se pronađu, odrede i prouče morfološke
karakteristike gljiva kao entomopatogena koji se javljaju u prirodnim populacijama tri vrste
potkornjaka (Curculionidae) – Ips sexdentatus Boern, Ips typographus (L.) i Dryocoetes autographus
(Ratz.).
Uginuli adulti su prikupljeni ispod kore stabala Pinus sylvestris i Picea abies poreklom sa
planina Meleševska i Vitoša. Patogene gljive su izolovane kao čiste kulture na podlozi SDAY
(Sabouraud dextrose agar with yeast extract) i određene na osnovu morfoloških osobina
kako na domaćinu, tako i u kulturi. Morfološke osobine izolata proučavane su fenotipskim
metodama.
Izolati gljiva dobijeni sa uginulih adulta Ips sexdentatus, Ips typographus i D. autographus
pripadaju vrstama Beauveria bassiana (Bals.-Criv.) Vuillemin, Beauveria brongniartii (Saccardo)
Petch i Isaria farinosa (Holmsk.) Fries (anamorf Ascomycota, Sordariomycetes: Hypocreales,
Cordycipitaceae). Dat je opis morfoloških osobina izolata.