Presence and Distribution of Economically Important Potato Viruses in Montenegro
Prisustvo i rasprostranjenost ekonomski znaèajnih virusa krompira u Crnoj Gori
Pregled/ Otvaranje
Autor
Zindovic, Jelena
Ključne reči
PotatoPotato viruses
PVYN/PVYNTN
PVYO
PVYC
DAS-ELISA
Krompir
virusi krompira
Metadata
Prikaz svih podataka o dokumentuApstrakt
The research was carried out, in the period 2002-2004 in order to determine the presence
and distribution of potato viruses at 12 different locations and on 9 different potato
varieties grown in Montenegro. The research included collecting of samples in seed potato
crops and testing of six economically important potato viruses: Potato leaf roll virus (PLRV),
Potato virus Y (PVY), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) i Potato virus
M (PVM). Using the direct enzyme-linked immunosorbent assay (DAS-ELISA) and commercial
antisera specific for six potato viruses, it was found that PVY was the most frequent virus
during the three-year research period. The second frequent virus was PVS, followed by PVA,
PLRV, PVM and PVX. Single and mixed infections were detected, and the most prevalent
were the single infections of PVY. Also, in the period 2002-2004, PVY had the highest distribution
and the number of present viruses was different at different localities and on different
potato varieties.
Further investigations were related to detailed characterization of the most prevalent
virus (PVY), which is at the same time economically the most important one. Serological
characterization of PVY was performed utilizing DAS-ELISA kit with commercial monoclonal
antibodies specific for detection of the three strain groups of PVY, and the two strain
groups - necrotic (PVYN/PVYNTN) and common (PVYO), were identified. Necrotic strains were
prevalent in 2002 and 2004, while in 2003 PVYO was the most frequent strain in virus population.
The presence of stipple streak strain (PVYC) was not detected in any of the tested
samples. U periodu od 2002. do 2004. godine obavljena su ispitivanja u cilju inventarizacije virusa
krompira na 12 različitih lokaliteta i kod 9 različitih sorti krompira u Crnoj Gori. Istraživanja
su obuhvatila sakupljanje uzoraka u usevima semenskog krompira i testiranje na
prisustvo šest ekonomski značajnih virusa krompira: virus uvijenosti lišća krompira (Potato
leaf roll virus, PLRV), virus crtičastog mozaika krompira ili Y virus krompira (Potato virus
Y, PVY), virus mozaika krompira ili X virus krompira (Potato virus X, PVX), S virus krompira
(Potato virus S, PVS), A virus krompira (Potato virus A, PVA) i M virus krompira (Potato virus
M, PVM). Primenom imunoenzimske metode na ploči (DAS-ELISA) i korišćenjem komercijalnih
antiseruma za šest virusa krompira, utvrđeno je dominantno prisustvo PVY tokom
sve tri godine ispitivanja. Drugi po zastupljenosti bio je PVS, zatim PVA, PLRV, PVM i PVX.
U testiranim uzorcima detektovano je prisustvo i pojedinačnih i mešovitih infekcija, pri
čemu je udeo pojedinačnih, među kojima PVY zaraza, u ukupnom broju testiranih uzoraka
bio dominantan. Takođe, utvrđeno je da je PVY bio najrašireniji virus u periodu od
2002. do 2004. godine, kao i da je broj ispitivanih virusa varirao po lokalitetima i sortama.
Dalja istraživanja odnosila su se na detaljnu karakterizaciju PVY kao najzastupljenijeg
i istovremeno ekonomski najznačajnijeg virusa krompira. U cilju serološke karakterizacije
PVY korišćena je DAS-ELISA metoda i monoklonalna antitela specifična za detekciju
tri grupe sojeva ovog virusa i tom prilikom utvrđeno je prisustvo nekrotičnih
(PVYN/PVYNTN) sojeva i običnog (PVYO) soja. Nekrotični sojevi su bili prevalentni u 2002.
i 2004. godini, dok je u 2003. godini veću zastupljenost u populaciji PVY imao obični
soj. Crtičasti soj (PVYC) nije dokazan ni u jednom ispitivanom uzorku tokom tri godine
istraživanja.