Potato cv. Romano reaction to primary and secondary infection with potato necrotic strain Y virus (PVYNTN)
Reakcija sorte Romano na primarne i sekundarne zaraze nekrotičnim sojem Y virusa krompira (PVYNTN)
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Author
Milosevic, Drago
Ristic, Danijela
Kuzmanovic, Slobodan
Starovic, Mira
Keywords
PotatoesPlant viruses
Potato Y potyvirus
Infection
Krompir
biljni virusi
Y virus krompira
zaraza
Metadata
Show full item recordAbstract
Primary and secondary infections with PVYNTN were investigated on forty plants of the
potato cv. Romano inoculated in a greenhouse in Serbia in 2012 and 2013. PVY isolates were
collected from the potato growing region of Čačak and identified by ELISA and RT-PCR
methods. The sequence of the Serbian isolate 3D (Acc. No. KJ946936) showed 100% match
with seven PVY isolates deposited in GenBank and described as NTN. A significant difference
was detected between PVYNTN symptoms exibited on leaves of the cv. Romano under
primary and secondary infections. The findings are significant because they are based on
symptoms observed, so that it is clear that there are two distinct types of infection: primary
and secondary. Symptoms of primary and secondary infection were the same on potato
tubers and had the form of necrotic rings. Upoređivani su simptomi primarnih i sekundarnih zaraza na četrdeset biljaka sorte
Romano inokulisanih izolatom PVYNTN u uslovima staklare, tokom 2012 i 2013 godine. Izolat
PVY je kolekcionisan u lokalitetu Čačak, identifikovan je ELISA testom i okarakterisan RT-PCR
metodom kao PVYNTN soj. Sekvenca CP gena odabranog izolata 3D (Acc. No. KJ946936) ispoljila
je 100% nukleotidnu identičnost sa sedam sekvenci PVY izolata deponovanih u GenBank
opisanih kao NTN soj. Ispoljeni simptomi na biljnoj masi primarno i sekundarno zaraženih
biljaka opisanim sojem su značajno različiti. Na osnovu ovih razlika može se zaključivati da li
su se biljke zarazile u tekućoj godini ili su sađene već zaražene krtole. Prstenasta nekroza je
zajednički simptom na krtolama primarno i sekundarno zaraženih biljaka.