Correlation of the Total Fenol Content with the Anti-Oxidative Activity of Red Wines from the Region of Banja Luka
Korelacija sadržaja ukupnih fenola sa antioksidativnom aktivnošću crvenih vina sa područja regije Banja Luka
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Author
Kukric, Zoran
Samelak, Ivan
Vucic, Goran
Vasilisin, Ladislav
Kukric, Novak
Keywords
red wineantioxidant activity
total phenols
DPPH
ABTS
Briggs- Raushers oscillatory reactions
crveno vino
antioksidativna aktivnost
ukupni fenoli
Briggs- Rausherove oscilatorne reakcije
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In this paper, the antioxidant activity of 3 red wine samples was determined using 1,1- diphenyl-2- picrylhydrazil (DPPH)- radical scavenging assay, 2,2’- azino- bis- (3- ethylbenzothiazoline-6- sulfonic acid) (ABTS) radical scavenging assay, so as Briggs- Raushers oscillatory reactions. Correlation of these antioxidant methods with the total phenol content in these red wine samples was also performed. The total phenol content was found to vary from 1790,67- 3209,97 mgGAE/Lwine. Antioxidant activity determined by DPPH method 15,45- 17,08 mmolTrolox/Lwine, ABTS method 23,21- 24,14 mmolTrolox/Lwine, and Briggs- Raushers oscillatory reactions 1,826- 3,848 mmolTrolox/Lwine respectively.
The total phenol content shows signifficant correlation with ABTS method (0,55), and very signifficant correlation with Briggs- Raushers oscillatory reactions (0,99) U ovom radu određena je antioksidativna aktivnost 3 uzorka crvenih vina primjenom gašenja 1,1- difenil- 2- pikrilhidrazil (DPPH)- stabilnog radikala, 2,2’- azino- bis- (3-etilbenzotiazolin- 6- sulfonska kiselina) (ABTS) stabilnog radikala, kao i Briggs- Rausherove oscilatorne reakcije. Urađena je i korelacija ovih antioksidativnih metoda sa sadržajem ukupnih fenola u navedenim uzorcima crvenih vina. Sadržaj ukupnih fenola se mijenjao u rasponu 1790,67- 3209,97 mgGAE/L. Antioksidativna aktivnost dobijena DPPH testom od 15,45- 17,08 mmolTrolox/L vina, ABTS testom od 23,21- 24,14 mmolTrolox/L vina, te Briggs - Rausherovom oscilatornom metodom 1,826- 3,848 mmolTrolox/Lvina respektivno.
Sadržaj ukupnih fenola pokazuje značajnu korelaciju sa ABTS testom (0.55), te vrlo visoku korelaciju sa Briggs- Rausherovim oscilatornim reakcijama (0.99).