Elaboration of Methods for Detection of Pseudomonas savastanoi pv. phaseolicola on Bean Seeds
Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja
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Author
Balaz, Jelica
Popovic, Tatjana
Vasic, Mirjana
Nikolic, Zorica
Keywords
Pseudomonas savastanoi pv. phaseolicolaBean
Seed
Artificial inoculation
ELISA
PCR
pasulj
seme
veštačka inokulacija
Metadata
Show full item recordAbstract
Pseudomonas savastanoi pv. phaseolicola detection on artificially inoculated bean seeds
was investigated. The method of the International Seed Federation – ISF (2006) was used.
It includes bacteria extraction from seeds, isolation on semiselective media and checking
the pathogenicity of investigated isolates. For verification of results, quick new methods of
investigation were used (ELISA test and PCR).
The results show that semiselective media MT (Milk Tween Agar) and MSP (Modified
Sucrose Peptone Agar) can be appropriate for isolation of this bacterium. Pathogenicity of the
investigated isolates was confirmed on cotyledon leaves of bean. ELISA test and PCR confirmed
that all investigated isolates and reisolates belong to the bacterium P. s. pv. phaseolicola. U radu je ispitivana mogućnost utvrđivanja prisustva bakterije Pseudomonas savastanoi
pv. phaseolicola sa veštački inokulisanog semena pasulja. Korišćena je metoda International
Seed Federation (ISF), koja uključuje ekstrakciju bakterija iz semena, izolaciju na poluselektivne
podloge i proveru patogenosti dobijenih izolata. U cilju potvrde rezultata korišćene su
brze savremene metode identifikacije (ELISA test i PCR).
Rezultati dobijeni tokom rada pokazuju da su poluselektivne podloge MT (Milk Tween
Agar) i MSP (Modified Sucrose Peptone Agar) pogodne za izolaciju ove bakterije. Patogenost
odabranih izolata dokazana je na kotiledonim listovima pasulja. Primenom ELISA testova i
PCR, potvrđeno je da svi ispitivani izolati i reizolati pripadaju bakteriji P. s. pv. phaseolicola.