Elaboration of Methods for Detection of Xanthomonas axonopodis pv. phaseoli on Bean Seeds
Razrada metoda za dokazivanje Xanthomonas axonopodis pv. phaseoli na semenu pasulja
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Author
Balaz, Jelica
Popovic, Tatjana
Vasic, Mirjana
Nikolic, Zorica
Keywords
Xanthomonas axonopodis pv. phaseoliBean
Seed
Artificial inoculation
ELISA
PCR
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Show full item recordAbstract
Xanthomonas axonopodis pv. phaseoli detection on artificially inoculated bean seeds
was investigated. The method of the International Seed Federation – ISF (2006) was used.
It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity
test of the investigated isolates. ELISA and PCR were used for verification of
results.
The results showed that the semiselective media MT (Milk Tween Agar) and XCP1
(Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli.
Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed
that all investigated isolates and reisolates belong to the bacterium X. a. pv. phaseoli. U radu je ispitivana mogućnost utvrđivanja prisustva bakterije Xanthomonas axonopodis
pv. phaseoli na veštački inokulisanom semenu pasulja. Ispitivanja su vršena po metodi
International Seed Federation (ISF), ekstrakcijom bakterija iz semena i izolacijom na poluselektivne
podloge uz proveru patogenosti dobijenih izolata. Za potvrdu dobijenih rezultata
korišćen je ELISA test i PCR.
Prema dobijenim rezultatima ova bakterija se može uspešno izolovati na poluselektivne
podloge MT (Milk Tween Agar) i XCP1 (Xanthomonas campestris pv. phaseoli Agar).
Patogenost je dokazana na mladim biljčicama pasulja. ELISA testovi i PCR su potvrdili da svi
ispitivani izolati i reizolati pripadaju bakteriji X. a. pv. phaseoli.