Raspberry bushy dwarf virus – a Grapevine Pathogen in Serbia

Abstract

During a field survey in 2005 in vineyards and grapevine nurseries at localities in central Serbia, a few plants with unusual virus-like symptoms were observed. Leaf samples were analyzed by DAS-ELISA for the presence of nine viruses. Besides other viruses frequently occurring on grapevine, Raspberry bushy dwarf virus (RBDV) was detected in two samples. Results were confirmed by nested-PCR and sequence analysis of the fragment in 5’ part of RNA-1. Obtained sequence shared at least 93% of nucleotide identity with the compared RBDV sequence originating from raspberry. The finding of Raspberry bushy dwarf virus on grapevine in Serbia is a second finding of this pathogen on grapevine worldwide. The first natural infection of grapevine with this virus was reported in Slovenia in 2003.

Pregledom vinograda i rastila vinove loze tokom 2005. godine u lokalitetima centralne Srbije na nekoliko biljaka su primećeni neuobičajeni simptomi koji su ukazivali na moguće prisustvo virusa. Uzorci listova su analizirani DAS-ELISA testom na prisustvo 9 virusa. Pored virusa koji se često javljaju na vinovoj lozi, kod dva uzorka je potvrđeno prisustvo virusa žbunaste kržljavosti maline (Raspberry bushy dwarf virus, RBDV). Nested-PCR metodom i sekvencionom analizom fragmenta iz 5’ regiona RNA-1 segmenta genoma RBDV potvrđeni su rezultati serološkog testa. Dobijena nukleotidna sekvenca pokazuje najmanje 93% sličnosti sa upoređenom sekvencom RBDV izolata iz maline. Potvrda prisustva RBDV na vinovoj lozi u Srbiji je drugi nalaz ovog patogena na vinovoj lozi u svetu. Prvi nalaz prirodne infekcije vinove loze ovim virusom saopšten je u Sloveniji 2003. godine.