Presence and Distribution of Economically Important Potato Viruses in Montenegro

Abstract

The research was carried out, in the period 2002-2004 in order to determine the presence and distribution of potato viruses at 12 different locations and on 9 different potato varieties grown in Montenegro. The research included collecting of samples in seed potato crops and testing of six economically important potato viruses: Potato leaf roll virus (PLRV), Potato virus Y (PVY), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) i Potato virus M (PVM). Using the direct enzyme-linked immunosorbent assay (DAS-ELISA) and commercial antisera specific for six potato viruses, it was found that PVY was the most frequent virus during the three-year research period. The second frequent virus was PVS, followed by PVA, PLRV, PVM and PVX. Single and mixed infections were detected, and the most prevalent were the single infections of PVY. Also, in the period 2002-2004, PVY had the highest distribution and the number of present viruses was different at different localities and on different potato varieties. Further investigations were related to detailed characterization of the most prevalent virus (PVY), which is at the same time economically the most important one. Serological characterization of PVY was performed utilizing DAS-ELISA kit with commercial monoclonal antibodies specific for detection of the three strain groups of PVY, and the two strain groups - necrotic (PVYN/PVYNTN) and common (PVYO), were identified. Necrotic strains were prevalent in 2002 and 2004, while in 2003 PVYO was the most frequent strain in virus population. The presence of stipple streak strain (PVYC) was not detected in any of the tested samples.

U periodu od 2002. do 2004. godine obavljena su ispitivanja u cilju inventarizacije virusa krompira na 12 različitih lokaliteta i kod 9 različitih sorti krompira u Crnoj Gori. Istraživanja su obuhvatila sakupljanje uzoraka u usevima semenskog krompira i testiranje na prisustvo šest ekonomski značajnih virusa krompira: virus uvijenosti lišća krompira (Potato leaf roll virus, PLRV), virus crtičastog mozaika krompira ili Y virus krompira (Potato virus Y, PVY), virus mozaika krompira ili X virus krompira (Potato virus X, PVX), S virus krompira (Potato virus S, PVS), A virus krompira (Potato virus A, PVA) i M virus krompira (Potato virus M, PVM). Primenom imunoenzimske metode na ploči (DAS-ELISA) i korišćenjem komercijalnih antiseruma za šest virusa krompira, utvrđeno je dominantno prisustvo PVY tokom sve tri godine ispitivanja. Drugi po zastupljenosti bio je PVS, zatim PVA, PLRV, PVM i PVX. U testiranim uzorcima detektovano je prisustvo i pojedinačnih i mešovitih infekcija, pri čemu je udeo pojedinačnih, među kojima PVY zaraza, u ukupnom broju testiranih uzoraka bio dominantan. Takođe, utvrđeno je da je PVY bio najrašireniji virus u periodu od 2002. do 2004. godine, kao i da je broj ispitivanih virusa varirao po lokalitetima i sortama. Dalja istraživanja odnosila su se na detaljnu karakterizaciju PVY kao najzastupljenijeg i istovremeno ekonomski najznačajnijeg virusa krompira. U cilju serološke karakterizacije PVY korišćena je DAS-ELISA metoda i monoklonalna antitela specifična za detekciju tri grupe sojeva ovog virusa i tom prilikom utvrđeno je prisustvo nekrotičnih (PVYN/PVYNTN) sojeva i običnog (PVYO) soja. Nekrotični sojevi su bili prevalentni u 2002. i 2004. godini, dok je u 2003. godini veću zastupljenost u populaciji PVY imao obični soj. Crtičasti soj (PVYC) nije dokazan ni u jednom ispitivanom uzorku tokom tri godine istraživanja.