Impact of Trichoderma spp. on Soybean Seed Germination and Potential Antagonistic Effect on Sclerotinia sclerotiorum
Uticaj Trichoderma spp. na klijavost semena soje i potencijalni antagonistički efekat na Sclerotinia sclerotiorum
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Author
Tancic, Sonja
Skrobonja, Jelica
Lalosevic, Mirjana
Jevtic, Radivoje
Vidic, Milos
Keywords
TrichodermaSeeds
Soybean
Germination
seme
soja
klijanje
Metadata
Show full item recordAbstract
Trichoderma species have been registered as species with important plant growth promoting
potential and antagonistic effect against various phytopathogens. Trichoderma isolates
originating from different soil types from the Vojvodina region (Serbia) were screened using
dual culture test for their antagonistic effect against the pathogen Sclerotinia sclerotiorum. All
tested isolates had high radial growth inhibition (RGI) factors of the pathogen and high colonization
percentage. Growth promoting activity of Trichoderma isolates on soybean seeds was
tested under glasshouse conditions. Soybean seeds were coated with suspensions of different
Trichoderma isolates and seed germination percentage, root and shoot length were measured.
According to data analysed in Statistica 10, using Duncan’s test, there were no significant
effects on shoot length among the tested isolates, compared to the control. The four tested
Trichoderma isolates showed significant positive effects on germination, root length and vigour
index, while two isolates exhibited no significant effect on any of the measured parameters. Vrste roda Trichoderma su poznate po svom antagonističkom efektu na veliki broj fitopatogenih
organizama kao i po stimulativnom efektu za rast biljke. Izolati Trichoderma spp.
testirani u ovom istraživanju su izolovani iz različitih tipova zemljišta u Vojvodini (Srbija). Test
dvojnih kultura je korišćen za testiranje antagonističkog dejstva izolata na patogena Sclerotinia
sclerotiorum. Svi testirani izolati su imali visok RGI (faktor inhibicije porasta micelije patogena)
i visok procenat kolonizacije patogena. Stimulativni efekat rasta Trichoderma izolata
na seme soje je testiran u uslovima staklenika, i sedmog dana su mereni dužina korena,
izdanka i klijavost semena tretiranih suspenzijom različitih izolata Trichoderma spp. Svi podaci
su analizirani primenom Duncan testa u programu Statistica 10, i ukazuju da nije bilo
statistički značajne razlike u dužini izdanaka tretiranih semena i kontrole. Četiri izolata su statistički
značajno uticala na povećanje dužine korena, klijavosti i vigor indeksa u odnosu na
kontrolu, dok dva izolata nisu statistički značajno uticala ni na jedan od merenih parametara.