Monitoring of Erwinia amylovora in Montenegro
Monitoring Erwinia amylovora u Crnoj Gori
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Author
Radunovic, Dragana
Gavrilovic, Veljko
Gasic, Katarina
Krstic, Marija
Keywords
Fire blightPome fruits
Hawthorn
Montenegro
Jabučasto voće
Bakteriozna plamenjača
Glog
Crna Gora
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Show full item recordAbstract
Recent studies of Erwinia amylovora in Montenegro, conducted from 2012 to 2014, indicated
that the bacterium was widespread in the northern, continental part of the country, where
the most important fruit-growing regions are situated. The presence of the bacterium was
confirmed on quince, pear, apple, medlar and hawthorn. Pathogenic, cultural and biochemical
characteristics of E. amylovora strains sampled from pome fruit species and indigenous flora
in Montenegro had been studied previously.
In the present study, serological tests were used for identification of E. amylovora strains
originating from pome fruit trees and indigenous plants. Monitoring of E. amylovora and
collection of samples with symptoms of bacterial fire blight from different hosts and locations
were performed in Montenegro from 2012 to 2014. Isolation of the bacterium on nutrient medium
produced a large number of isolates, whose pathogenicity was confirmed on immature pear
fruits. Twenty-seven strains of the bacterium, originating from three pome fruit species (quince,
pear and apple) and one indigenous species (hawthorn) were selected for serological analyses.
Two applied serological methods, ELISA and IF test, enabled rapid detection of the bacterium and
simultaneous examination of a large number of samples over a short period of time. Serological
analyses showed high homogeneity in antigenic structure of the studied E. amylovora strains
sampled from quince, pear, apple and hawthorn from nine locations in Montenegro. Novija proučavanja Erwinia amylovora u Crnoj Gori, sprovedena u periodu od 2012 do
2014. godine, pokazuju da je ova bakterija široko rasprostranjena u severnom, kontinentalnom
delu zemlje, gde se nalaze i najznačajniji voćarski regioni. Prisustvo bakterije potvrđeno je na
dunji, krušci, jabuci, mušmuli i glogu. U prethodnim istraživanjima proučene su patogene,
odgajivačke i biohemijske odlike sojeva E. amylovora poreklom sa jabučastih voćnih vrsta i
biljaka spontane flore u Crnoj Gori.
U ovom radu primenjeni su serološki testovi u identifikaciji sojeva E. amylovora poreklom
sa jabučastih voćaka i biljaka spontane flore. Monitoring E. amylovora i sakupljanje uzoraka
sa simptomima bakteriozne plamenjače izvršeno je u periodu od 2012 do 2014. godine, sa
različitih domaćina i lokaliteta u Crnoj Gori. Izolacijom bakterije na hranljive podloge dobijen
je veći broj izolata, čija je patogenost potvrđena na zelenim plodovima kruške. Za serološke
analize odabrano je 27 sojeva ove bakterije, poreklom sa tri jabučaste voćne vrste (dunja,
kruška i jabuka) i jedne vrste iz spontane flore (glog). Primenjene su dve serološke metode:
ELISA i IF test, koje su omogućile brzu detekciju bakterije i istovremeno ispitivanje velikog
broja uzoraka za kratko vreme. Serološkim analizama utvrđena je visoka homogenost u
antigenoj strukturi proučavanih sojeva E. amylovora poreklom sa dunje, kruške, jabuke i
gloga, iz 9 lokaliteta u Crnoj Gori.