Similarity of Sugar Components in Waste Buckwheat Flour Determined Using GC-MS

Abstract

Gas chromatography with mass spectrometry (GC-MS) was used for performing a qualitative analysis of the hydrosoluble flour extracts of 9 buckwheat samples, taken as leftovers from restaurants. All 9 samples were first defatted with hexane. Samples of defatted flour were dried in the air and then extracted with ethanol. Ethanol extracts were used for the analysis of soluble carbohydrates. TMSi (trimethylsilylimidazole) was used as a reagent for the derivatization of carbohydrates into trimethylsilyl-ethers. The results show that the dominant trimethylsilyl-ethers of sugars are actually very similar in all buckwheat samples. The following cluster analysis was used for the comparison of the hydrosoluble extracts of 9 waste buckwheat flour samples.

Kvalitativna analiza hidrosolubilnih komponenti 9 uzoraka otpadnog brašna heljde izvedena je pomoću gasne hromatografije sa masenom spektrometrijom (GC-MS). Svi uzorci prethodno su obezmašćeni heksanom. Uzorci obezmašćenog brašna osušeni su na vazduhu, nakon čega je izvršena ekstrakcija etanolom. Etanolni ekstrakti upotrebljeni su za analizu rastvorljivih ugljenih hidrata. Kao reagens za derivatizaciju ugljenih hidrata u metilsilil-etre korišćen je TMSi (trimetilsililimidazol). Rezultati pokazuju da je sastav najzastupljenijih ugljenih hidrata, odnosno njihovih trimetilsilil-etara, veoma sličan u svih 9 uzoraka heljde. U cilju poređenja hidrosolubilnog sastava etanolnih ekstrakata uzoraka otpadnog brašna heljde primenjena je klaster analiza.