Genotoxic Effect of the Cotinus Coggygria Scop. Methanol Extract on Drosophila Melanogaster

Abstract

Plant extracts that appear to have favorable properties, may contain chemical compounds with mutagenic, teratogenic and/or carcinogenic activity, and it is of great importance to the inclusion of genotoxic approaches to toxicological evaluation of plant extracts. Using a comet assay on eukaryotic model organism Drosophila melanogaster in in vivo condition, potential genotoxic activity of the methanol extract of plant Cotinus coggygria Scop. was determined. Treatment with the methanol extracts, at a concentration of 1%, caused no significant changes compared to the negative control. Based on the distribution of comet class and selected quantitative parameters (% DNA in tail and tail length) it can be concluded that a methanol extract obtained from C. coggygria at a concentration of 1% does not shows genotoxic activity.

Uključivanje genotoksičnog pristupa u toksikološku evaluaciju biljnih ekstrakata, koji i pored povoljnih svojstava mogu da sadrže komponente sa mutagenim, teratogenim i/ili kancerogenim aktivnostima, je od velike važnosti. Primenom Komet testa kod eukariotskog model organizma Drosophila melanogaster u in vivo uslovima ispitivana je genotoksična aktivnost metanolskog ekstrakta biljke Cotinus coggygria Scop. Тretman sa ekstraktom u koncentraciji od 1% nije uzrokovao statistički značajne promene u odnosu na negativnu kontrolu. Na osnovu raspodela komet klasa i odabranih kvantitativnih parametara može se zaključiti da ekstrakt biljke C. coggygria ne pokazuje genotoksičnu aktivnost.