Genotoxic Effect of the Cotinus Coggygria Scop. Methanol Extract on Drosophila Melanogaster
Genotoksični efekat metanolskog ekstrakta biljke Cotinus Coggygria Scop. kod Drosophila Melanogaster
Author
Matic, Sanja
Stanic, Snezana
Mladenovic, Milan
Stankovic, Nevena
Keywords
Comet assayDrosophila melanogaster
Cotinus coggygria Scop.
Komet test
Metadata
Show full item recordAbstract
Plant extracts that appear to have favorable properties, may contain chemical
compounds with mutagenic, teratogenic and/or carcinogenic activity, and it is of great
importance to the inclusion of genotoxic approaches to toxicological evaluation of plant
extracts. Using a comet assay on eukaryotic model organism Drosophila melanogaster
in in vivo condition, potential genotoxic activity of the methanol extract of plant Cotinus
coggygria Scop. was determined. Treatment with the methanol extracts, at a
concentration of 1%, caused no significant changes compared to the negative control.
Based on the distribution of comet class and selected quantitative parameters (% DNA
in tail and tail length) it can be concluded that a methanol extract obtained from C.
coggygria at a concentration of 1% does not shows genotoxic activity. Uključivanje genotoksičnog pristupa u toksikološku evaluaciju biljnih
ekstrakata, koji i pored povoljnih svojstava mogu da sadrže komponente sa mutagenim,
teratogenim i/ili kancerogenim aktivnostima, je od velike važnosti. Primenom Komet testa
kod eukariotskog model organizma Drosophila melanogaster u in vivo uslovima
ispitivana je genotoksična aktivnost metanolskog ekstrakta biljke Cotinus coggygria Scop.
Тretman sa ekstraktom u koncentraciji od 1% nije uzrokovao statistički značajne promene
u odnosu na negativnu kontrolu. Na osnovu raspodela komet klasa i odabranih
kvantitativnih parametara može se zaključiti da ekstrakt biljke C. coggygria ne pokazuje
genotoksičnu aktivnost.