Fourier Transform – Near Infrared Spectroscopy for Rapid and Nondestructive Measurement of Amylose Content of Paddy

Abstract

The quality and quantity of amylose are two important factors that determine the quality of paddy. The feasibility of developing a technique for rapid monitoring of paddy quality by using Fourier Transform - Near Infrared (FT-NIR) Spectroscopy combined with chemometrics was investigated. Spectra of 250 paddy samples were collected by using an integrating sphere accessory. The amylose content was analyzed by reference method. It ranged from 13.24 to 27.93%. The spectral data was analyzed by partial least squares (PLS) algorithm and calibration model generated. Correlation coefficient (R2) for the calibration model was >0.78 with root mean square error of estimation (RMSEE) < 1.8. The validation model had R2> 0.72 and root mean square error of cross-validation (RMSECV) < 2. FT-NIR spectroscopy identified samples containing amylose at 5184 and 6834 cm-1 wave number. A fast, simple and accurate method to quantify the amylose of paddy was developed by using FT-NIR spectroscopy.

Kvalitet i kvantitet amiloze su dva značajna faktora koja određuju kvalitet pirinča. Ovede je razvijena tehnika brzog merenja kvaliteta pirinča upotrebom Furijeove transformacije – spektroskopije u bliskoj IR oblasti kombinovane sa hemometrijom. Analiziran je spektar 250 uzoraka pirinča integrisanim svernim uređajem. Sadržaj amiloze iznosio je 13.24 do 27.93%. Spektralni podaci su analizirani algoritmom parcijalnih najmanjijh kvadrata i generisan je kalibracioni model. Koeficijent korelacije bio je >0.78 sa srednjim kvadratom odstupanja < 1.8. Validacioni model imao je R2> 0.72 i srednji kvadrat odstupanja unakrsne procene < 2. FT-NIR spektroskopijom su identifikovani uzorci koji sadrže amilozu sa talasnim brojevima 5184 i 6834 cm-1. Tako je razvijen brz, jednostavan i pouzdan metod određivanja sadržaja amiloze.