Respiration Rate Models of Fresh Chickpea Sprouts (Cicer Arietinum L.) under Modified Atmosphere Packaging

Abstract

The closed system glass container headspace gaseous composition, in terms of O2 and CO2 concentrations, in modified atmosphere packaging (MAP) of Chickpea Sprouts (Cicer arietinum L.) at 5, 10, 15, 20 and 25oC temperature were respectively monitored within 5h by a O2/CO2 gas analyzer. The respiration rate of Chickpea sprouts under modified atmosphere packaging at any instant of time were calculated by a C+ program based on closed system. The dependence of respiration rate on gas composition was well described by a Michaelis-Menten type enzyme kinetic model equation with uncompetitive type of mixed CO2 inhibition. The model parameters were estimated by fitting the model to the experimental data by non-linear regression using GraphPad Prism 5. The relationship between enzyme kinematics model parameters and temperature was well described by the Arrhenius equation. The results suggest that, the respiration rate of Chickpea sprouts as well as kinematic parameters increased with temperature. Results of the study can be applied to the MAP design for Chickpea sprouts.

Sastav gasova na vrhu staklenog kontejnera u zatvorenom sistemu, uključujući koncentracije O2 i CO2, u modifikovanom atmosverskom pakovanju (MAP) klica leblebija (Cicer arietinum L.) pri temperaturama od 5, 10, 15, 20 i 25oC je praćen pojedinačno u periodima od po 5 časova O2/CO2 gas analizatorom. Stopa respiracije svežih klica leblebija u modifikovanom atmosverskom pakovanju proračunavana je u realnom vremenu C+ programom zasnovanim na zatvorenom sistemu. Zavisnost stope respiracije od sastava gasova je dobro opisana jednačinom Michaelis-Menten tipa enzimskog kinetičkog modela sa nekonkurentnim tipom inhibicije izmešanog CO2. Parametri modela su procenjeni prilagođavanjem modela eksperimentalnim podacima primenom nelinearne regresije u GraphPad Prism 5. Odnos između parametara modela kinematike enzima i temperature bila je dobro pisana Arrhenius jednačinom. Rezultati ukazuju na porast stope respiracije klica leblebija i kinematičkih parametara sa porastom temperature. Rezultati studije mogu se primeniti na proračun MAP za klice leblebija.