Morfophysiological And Hematological Features Of Recovery–Maternal Stock Of Sturgeon Fish Rearing In Sturgeon Fish Hatchery Of Azerbaijan
Morfološki i hematološki pokazatelji matičnog jata za obnovu jesetarskih riba gajenih u jesetarskim mrestilištima u Azerbejdžanu
Author
Mamedov, Ch.A.
Keywords
Persian sturgeonrecovery-maternal stock
erythrocyte
leukocyte
lymphocytes
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In Azerbaijan the forming of recovery-maternal stock of sturgeon fish is carrying out since 2004 year and breeding of fish from the stage of larvae till the adult conditions is fulfilled by using of pool method. Meanwhile it is known that quality of a breeding material in many respects depends on conditions of their maintenance. The task of our researches included studying on the basis of mofophysiological and hematological parameters to the extent of forming of the major functional systems of uneven-aged species of recovery-maternal stock of sturgeon fish during their long growing in bondage.
Persian sturgeon (Acipenser persicus) of ages 1 to 5 served as study objects in 2009. Directly after collecting blood from a tail vein content of hemoglobin and ESR (erythrocyte sedimentation rate) were defined, and also smear of blood samples were made by classical technique of Romanovsky-Gimza. Calculation of blood corpuscles was carried out in chamber of Goryayeva with staining blood cells by neutral read and crystal violet. 200 leukocyte cells were counted in each test. For differentiation of cells of the red blood 500 erythrocytes of different age groups of experimental fish were counted on smear. Index of leucocytes shift is determined according to commonly accepted methodology.
As researches have shown, on the first to year of a life of young fish of the Persian sturgeon erythropoiesis is generated completely. It has been defined that mature erythrocytes (76,5 - 81,5 %) prevail in peripheral blood at all age groups of young fish of researched kind. Ratio of basophilic normoblasts has comprised 0,8-1,4%, and hemolyzed erythrocytes 0,4-1,6 %. The insignificant number of pathological forms of erythrocytes, in our opinion, testifies to natural dying off of cells of erythrocyte series.
The structure of blood corpuscle of uneven-aged species of repair broodstock of the Persian sturgeon, grown up on industrial conditions, testifies that function of leukopoiesis is generated at them. Lymphoid nature of leukocytes was determined in researched fish of Persian sturgeon which was reared in the pool conditions of Sturgeon Fish Hatchery. The obtained information about the right (lymphoid) shift in leukogram of the blood of the uneven-aged young fish witnesses the normal developing leukopoiesis. Index of leucocytes shift has a direct dependence on the age of reared fish: yearlings – 0,54; two-year old – 0,40; three-year old – 0,38; four year old 0,37 and five-year old– 0,32.
Making preliminary conclusions of hematological researches of the Persian sturgeon as the modeling object used for formation of recovery-maternal stock on Khilly Sturgeon Fish Hatchery, it is possible to draw following conclusions: - 1. In process of growth of young Persian sturgeon the number of erythrocytes on unit of volume of blood increases; 2. On the first year of a life of young fish of the Persian sturgeon the erythropoiesis is generated completely; 3. The number of leukocytes on unit of volume of blood of the Persian sturgeon has seasonal variability and has no direct dependence on age of fish; 4. The quantity of immune cells (lymphocytes) has direct dependence on age of fishes.