Biochemical Composition of Culture Filtrate of the Fungus Colletotrichum Lini – Anthracnose Pathogen Flax
Author
Proliotova, N.V.
Keywords
anthracnoseflax
culture filtrate
existing proteins
amino acid
cysteine
serine
glutamine
asparagines
arginine
tyrosine
Metadata
Show full item recordAbstract
Anthracnose occurs in flax seedlings annually and everywhere. Fungus
Colletotrichum lini acts on the plant by the virtue of toxins. For the development of
selective media in the process of anthracnose resistant cell selection in vitro anthracnose
pathogen culture filtrate is used. The diversity and specificity of the existing proteins in
nature are determined by amino acid composition and genetically precisely defined
sequence of amino acids in the polypeptide chain. Qualitative analysis of free amino
acids revealed that there are the following free amino acids in the culture filtrate strains
419 and 639: on the 7th day culture filtrate cysteine Rf = 0,07 presented in both strains;
on the 14th day serine Rf = 0,22 was defined in both strains ; on the 21st day - glutamine
Rf = 0,17; on the 28th day - asparagine Rf = 0,12; on the 35th day - arginine Rf = 0,14;
on the 42nd day - tyrosine Rf = 0,44.