Effect of Rooster Macrophages on the Motility Characteristics of Spermatozoa
Author
Kuzelova, L
Chrenek, P
Keywords
CASAmacrophages
motility
rooster
spermatozoa
Metadata
Show full item recordAbstract
The aim of our study was to detect macrophages in rooster semen and subsequently to asses their effect on the spermatozoa motility parameters. Semen samples were collected from Ross PM3 breeder males (n = 24) by the dorso-abdominal massage into prepared sterile tube. Macrophages were identified using fluorescent dye Alexa Fluor-AcLDL and evaluated under a Leica fluorescent microscope (Leica Microsystem, Germany). Roosters (n=8) with the occurrence of macrophages above 20% per samples were classified to the group R1 and roosters (n=16) with the occurrence of macrophages 0-10% were classified to the group R2. The mean content of macrophages was 23.43±1.82 vs. 7.59±1.39 (group 1 vs. group 2, P<0.001) during experiment. The concentration (109 per ml), percentage of motile spermatozoa (motility > 5 µm/s) and percentage of progressive motile spermatozoa (motility > 20 µm/s) of two heterospermic samples were measured using computer-assisted sperm analysis (CASA system, Sperm Vision™). We observed significantly (P<0.05) lower progressive motile of rooster spermatozoa in the group R1 in comparison to group R2 (25.04±5.98 vs. 46.38±3.28). Based on the observed preliminary results, we hypothesized that the higher presence of macrophages in semen may have a negative effect on the quality of rooster spermatozoa in terms of their motility. Further experiments are required in order to prove our hypothesis.